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Tel Test Inc rna-stat60 total rna isolation reagent
Rna Stat60 Total Rna Isolation Reagent, supplied by Tel Test Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rna+stat60/rna+stat+60/pm40347464-121-23-28
Average 90 stars, based on 1 article reviews
rna-stat60 total rna isolation reagent - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Gene Expression:

Article Title: Derivation of transplantable human thyroid follicular epithelial cells from induced pluripotent stem cells
Article Snippet: .. 110 Gene expression 111 Total RNA was extracted from unsorted and sorted cell samples using RNA Stat60 (Tel-Test Inc.) 112 or RNeasy Plus Mini Kit (QIAGEN) and cDNA was synthesized by reverse transcription with 113 SuperScript VILO cDNA Synthesis kit (Invitrogen) or high-capacity cDNA Reverse Transcription 114 kit (Applied biosystems). .. Real-Time quantitative PCR was performed for 40 cycles using TaqMan 115 Fast Universal PCR Master Mix (Applied Biosystems) or TaqMan Universal PCR Master Mix 116 (Applied Biosystems) and the following TaqMan probes (Applied Biosystems): Brachyury [T] 117 (Hs00610080_m1), FOXA2 (Hs00232764_m1), FOXE1 (Hs00916085_s1), HHEX 118 (Hs00242160_m1), NKX2-1 (Hs00968940_m1), OCT4 [POU5F1] (Hs00999632_g1), PAX8 119 (Hs01015257_g1), SLC5A5 (Hs00166567_m1), SOX2 (Hs01053049_s1), SOX17 120 (Hs06634937_s1), TG (Hs00174974_m1), TPO (Hs00892519_m1), and TSHR 121 (Hs01053846_m1).

Synthesized:

Article Title: Derivation of transplantable human thyroid follicular epithelial cells from induced pluripotent stem cells
Article Snippet: .. 110 Gene expression 111 Total RNA was extracted from unsorted and sorted cell samples using RNA Stat60 (Tel-Test Inc.) 112 or RNeasy Plus Mini Kit (QIAGEN) and cDNA was synthesized by reverse transcription with 113 SuperScript VILO cDNA Synthesis kit (Invitrogen) or high-capacity cDNA Reverse Transcription 114 kit (Applied biosystems). .. Real-Time quantitative PCR was performed for 40 cycles using TaqMan 115 Fast Universal PCR Master Mix (Applied Biosystems) or TaqMan Universal PCR Master Mix 116 (Applied Biosystems) and the following TaqMan probes (Applied Biosystems): Brachyury [T] 117 (Hs00610080_m1), FOXA2 (Hs00232764_m1), FOXE1 (Hs00916085_s1), HHEX 118 (Hs00242160_m1), NKX2-1 (Hs00968940_m1), OCT4 [POU5F1] (Hs00999632_g1), PAX8 119 (Hs01015257_g1), SLC5A5 (Hs00166567_m1), SOX2 (Hs01053049_s1), SOX17 120 (Hs06634937_s1), TG (Hs00174974_m1), TPO (Hs00892519_m1), and TSHR 121 (Hs01053846_m1).

Article Title: Osteoblast-derived FGF9 regulates skeletal homeostasis
Article Snippet: .. Frozen tissues were pulverized using a biopulverizer (Biospec Products, Inc., Bartlesville, OK, USA), followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using Micro-to-Midi Total RNA Purification Kit (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer. .. Gene amplification was measured with SYBR Green using the ABI Prism ViiA TM 7 real-time PCR System (Waltham, MA, USA).

Article Title: Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
Article Snippet: Cell lysates were normalized by concentration and run through an SDS‐Page gel followed by immunoblot analysis with specific antibodies against perilipin 1, pan acetylysine (Cell Signaling Technology), adiponectin Thermo Scientific # PA1‐054), Sirt3 (as described ), and β‐actin (A1978, Clone AC‐15, Sigma). .. Mature differentiated adipocytes from ST2 cells were harvested, followed by RNA extraction using RNA STAT60 (Tel‐Test, Inc, Friendswood, Texas) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA USA). cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc, Foster City, California, USA) and random hexamer primers according to the recommendations of the manufacturer. ..

Reverse Transcription:

Article Title: Derivation of transplantable human thyroid follicular epithelial cells from induced pluripotent stem cells
Article Snippet: .. 110 Gene expression 111 Total RNA was extracted from unsorted and sorted cell samples using RNA Stat60 (Tel-Test Inc.) 112 or RNeasy Plus Mini Kit (QIAGEN) and cDNA was synthesized by reverse transcription with 113 SuperScript VILO cDNA Synthesis kit (Invitrogen) or high-capacity cDNA Reverse Transcription 114 kit (Applied biosystems). .. Real-Time quantitative PCR was performed for 40 cycles using TaqMan 115 Fast Universal PCR Master Mix (Applied Biosystems) or TaqMan Universal PCR Master Mix 116 (Applied Biosystems) and the following TaqMan probes (Applied Biosystems): Brachyury [T] 117 (Hs00610080_m1), FOXA2 (Hs00232764_m1), FOXE1 (Hs00916085_s1), HHEX 118 (Hs00242160_m1), NKX2-1 (Hs00968940_m1), OCT4 [POU5F1] (Hs00999632_g1), PAX8 119 (Hs01015257_g1), SLC5A5 (Hs00166567_m1), SOX2 (Hs01053049_s1), SOX17 120 (Hs06634937_s1), TG (Hs00174974_m1), TPO (Hs00892519_m1), and TSHR 121 (Hs01053846_m1).

Article Title: Osteoblast-derived FGF9 regulates skeletal homeostasis
Article Snippet: .. Frozen tissues were pulverized using a biopulverizer (Biospec Products, Inc., Bartlesville, OK, USA), followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using Micro-to-Midi Total RNA Purification Kit (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer. .. Gene amplification was measured with SYBR Green using the ABI Prism ViiA TM 7 real-time PCR System (Waltham, MA, USA).

Article Title: Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
Article Snippet: Cell lysates were normalized by concentration and run through an SDS‐Page gel followed by immunoblot analysis with specific antibodies against perilipin 1, pan acetylysine (Cell Signaling Technology), adiponectin Thermo Scientific # PA1‐054), Sirt3 (as described ), and β‐actin (A1978, Clone AC‐15, Sigma). .. Mature differentiated adipocytes from ST2 cells were harvested, followed by RNA extraction using RNA STAT60 (Tel‐Test, Inc, Friendswood, Texas) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA USA). cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc, Foster City, California, USA) and random hexamer primers according to the recommendations of the manufacturer. ..

cDNA Synthesis:

Article Title: Derivation of transplantable human thyroid follicular epithelial cells from induced pluripotent stem cells
Article Snippet: .. 110 Gene expression 111 Total RNA was extracted from unsorted and sorted cell samples using RNA Stat60 (Tel-Test Inc.) 112 or RNeasy Plus Mini Kit (QIAGEN) and cDNA was synthesized by reverse transcription with 113 SuperScript VILO cDNA Synthesis kit (Invitrogen) or high-capacity cDNA Reverse Transcription 114 kit (Applied biosystems). .. Real-Time quantitative PCR was performed for 40 cycles using TaqMan 115 Fast Universal PCR Master Mix (Applied Biosystems) or TaqMan Universal PCR Master Mix 116 (Applied Biosystems) and the following TaqMan probes (Applied Biosystems): Brachyury [T] 117 (Hs00610080_m1), FOXA2 (Hs00232764_m1), FOXE1 (Hs00916085_s1), HHEX 118 (Hs00242160_m1), NKX2-1 (Hs00968940_m1), OCT4 [POU5F1] (Hs00999632_g1), PAX8 119 (Hs01015257_g1), SLC5A5 (Hs00166567_m1), SOX2 (Hs01053049_s1), SOX17 120 (Hs06634937_s1), TG (Hs00174974_m1), TPO (Hs00892519_m1), and TSHR 121 (Hs01053846_m1).

Article Title: EphB2 receptor promotes dermal fibrosis in systemic sclerosis.
Article Snippet: .. RNA extraction, cDNA synthesis and qPCR Total RNA was extracted using RNA Stat60® (Tel-Test Inc, USA) following standard phenol-chloroform procedure, and further purified using the NucleoSpin RNA-II purification kit (Machery-Nagel) following the manufacturer’s instructions. .. Complementary DNAs were generated by reverse transcription using Superscript reverse transcriptase-II (Invitrogen, USA) as previously described (21).

RNA Extraction:

Article Title: Osteoblast-derived FGF9 regulates skeletal homeostasis
Article Snippet: .. Frozen tissues were pulverized using a biopulverizer (Biospec Products, Inc., Bartlesville, OK, USA), followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using Micro-to-Midi Total RNA Purification Kit (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer. .. Gene amplification was measured with SYBR Green using the ABI Prism ViiA TM 7 real-time PCR System (Waltham, MA, USA).

Article Title: EphB2 receptor promotes dermal fibrosis in systemic sclerosis.
Article Snippet: .. RNA extraction, cDNA synthesis and qPCR Total RNA was extracted using RNA Stat60® (Tel-Test Inc, USA) following standard phenol-chloroform procedure, and further purified using the NucleoSpin RNA-II purification kit (Machery-Nagel) following the manufacturer’s instructions. .. Complementary DNAs were generated by reverse transcription using Superscript reverse transcriptase-II (Invitrogen, USA) as previously described (21).

Article Title: Triggering Receptor Expressed on Myeloid Cells-1 (TREM-1) Contributes to Bordetella pertussis Inflammatory Pathology.
Article Snippet: .. Lung tissue was harvested directly into RNAlater (Sigma-Aldrich) and stored overnight at 4°C before RNA extraction using RNA Stat60 (TelTest, Inc.) as per the manufacturer’s instructions. ..

Article Title: Sirt3 Pharmacologically Promotes Insulin Sensitivity through PI3/AKT/mTOR and Their Downstream Pathway in Adipocytes
Article Snippet: Secondary antibody anti-rabbit antibody (Cat#7074, Cell Signaling Technology) or anti-mouse anti-body (Cat#7076, Cell Signaling Technology) were used. .. Cells were harvested, followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA, USA). .. RNA extraction protocol was followed to isolate mRNA. cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer.

Article Title: Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
Article Snippet: Cell lysates were normalized by concentration and run through an SDS‐Page gel followed by immunoblot analysis with specific antibodies against perilipin 1, pan acetylysine (Cell Signaling Technology), adiponectin Thermo Scientific # PA1‐054), Sirt3 (as described ), and β‐actin (A1978, Clone AC‐15, Sigma). .. Mature differentiated adipocytes from ST2 cells were harvested, followed by RNA extraction using RNA STAT60 (Tel‐Test, Inc, Friendswood, Texas) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA USA). cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc, Foster City, California, USA) and random hexamer primers according to the recommendations of the manufacturer. ..

Purification:

Article Title: Osteoblast-derived FGF9 regulates skeletal homeostasis
Article Snippet: .. Frozen tissues were pulverized using a biopulverizer (Biospec Products, Inc., Bartlesville, OK, USA), followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using Micro-to-Midi Total RNA Purification Kit (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer. .. Gene amplification was measured with SYBR Green using the ABI Prism ViiA TM 7 real-time PCR System (Waltham, MA, USA).

Article Title: EphB2 receptor promotes dermal fibrosis in systemic sclerosis.
Article Snippet: .. RNA extraction, cDNA synthesis and qPCR Total RNA was extracted using RNA Stat60® (Tel-Test Inc, USA) following standard phenol-chloroform procedure, and further purified using the NucleoSpin RNA-II purification kit (Machery-Nagel) following the manufacturer’s instructions. .. Complementary DNAs were generated by reverse transcription using Superscript reverse transcriptase-II (Invitrogen, USA) as previously described (21).

Article Title: Sirt3 Pharmacologically Promotes Insulin Sensitivity through PI3/AKT/mTOR and Their Downstream Pathway in Adipocytes
Article Snippet: Secondary antibody anti-rabbit antibody (Cat#7074, Cell Signaling Technology) or anti-mouse anti-body (Cat#7076, Cell Signaling Technology) were used. .. Cells were harvested, followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA, USA). .. RNA extraction protocol was followed to isolate mRNA. cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer.

Article Title: Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
Article Snippet: Cell lysates were normalized by concentration and run through an SDS‐Page gel followed by immunoblot analysis with specific antibodies against perilipin 1, pan acetylysine (Cell Signaling Technology), adiponectin Thermo Scientific # PA1‐054), Sirt3 (as described ), and β‐actin (A1978, Clone AC‐15, Sigma). .. Mature differentiated adipocytes from ST2 cells were harvested, followed by RNA extraction using RNA STAT60 (Tel‐Test, Inc, Friendswood, Texas) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA USA). cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc, Foster City, California, USA) and random hexamer primers according to the recommendations of the manufacturer. ..

Random Hexamer:

Article Title: Osteoblast-derived FGF9 regulates skeletal homeostasis
Article Snippet: .. Frozen tissues were pulverized using a biopulverizer (Biospec Products, Inc., Bartlesville, OK, USA), followed by RNA extraction using RNA STAT60 (Tel-Test, Inc., Friendswood, TX, USA) and subsequent purification using Micro-to-Midi Total RNA Purification Kit (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using TaqMan Reverse Transcription Reagents (Applied Biosystems, Inc., Foster City, CA, USA) and random hexamer primers according to the recommendations of the manufacturer. .. Gene amplification was measured with SYBR Green using the ABI Prism ViiA TM 7 real-time PCR System (Waltham, MA, USA).

Article Title: Sirt3 regulates adipogenesis and adipokine secretion via its enzymatic activity
Article Snippet: Cell lysates were normalized by concentration and run through an SDS‐Page gel followed by immunoblot analysis with specific antibodies against perilipin 1, pan acetylysine (Cell Signaling Technology), adiponectin Thermo Scientific # PA1‐054), Sirt3 (as described ), and β‐actin (A1978, Clone AC‐15, Sigma). .. Mature differentiated adipocytes from ST2 cells were harvested, followed by RNA extraction using RNA STAT60 (Tel‐Test, Inc, Friendswood, Texas) and subsequent purification using PureLink RNA Mini Kit (ThermoFisher Scientific, Waltham, MA USA). cDNA was synthesized using TaqMan Reverse Transcription reagents (Applied Biosystems, Inc, Foster City, California, USA) and random hexamer primers according to the recommendations of the manufacturer. ..

Real-time Polymerase Chain Reaction:

Article Title: EphB2 receptor promotes dermal fibrosis in systemic sclerosis.
Article Snippet: .. RNA extraction, cDNA synthesis and qPCR Total RNA was extracted using RNA Stat60® (Tel-Test Inc, USA) following standard phenol-chloroform procedure, and further purified using the NucleoSpin RNA-II purification kit (Machery-Nagel) following the manufacturer’s instructions. .. Complementary DNAs were generated by reverse transcription using Superscript reverse transcriptase-II (Invitrogen, USA) as previously described (21).

Quantitative RT-PCR:

Article Title: Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver
Article Snippet: .. Cholesterol and triglycerides were then expressed as mg/g of liver tissue. qRT-PCR Analysis: RNA-STAT60 was used to homogenize liver tissues and total RNA was isolated (catalog: CS-502; Tel-test Inc.; Friendswood, TX, USA). .. RNA quantity and purity was assessed with a Nanodrop OneC spectrometer (Thermo Scientific) (catalog: 701-058112; Madison, MI, USA).

Isolation:

Article Title: Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver
Article Snippet: .. Cholesterol and triglycerides were then expressed as mg/g of liver tissue. qRT-PCR Analysis: RNA-STAT60 was used to homogenize liver tissues and total RNA was isolated (catalog: CS-502; Tel-test Inc.; Friendswood, TX, USA). .. RNA quantity and purity was assessed with a Nanodrop OneC spectrometer (Thermo Scientific) (catalog: 701-058112; Madison, MI, USA).



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